DNA

Part:BBa_K4051016:Design

Designed by: Clodagh Healy   Group: iGEM21_MTU-CORK   (2021-10-08)


Cld


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 452
    Illegal BsaI.rc site found at 734
    Illegal SapI.rc site found at 614


Design Notes

codon optimised for E.coli


Source

Dechloromonas (Azospira) suillum locus Dsui_0145

Genbank Accession: CP003153.1

Generic Histag used

Generic Secretion Signal from Gaussian luciferase


References