RNA
Part:BBa_K4050019
Designed by: Kelly Styles Group: iGEM21_Victoria_Wellington (2021-09-30)
20 nt spacer sgRNA for spermidine synthase gene in S. elongatus UTEX 2973
20 nt spacer for an sgRNA to recruit Cas9 to the spermidine synthase gene (speE - CP006471.1:2636619..2637479) in Synechococcus elongatus UTEX 2973 to perform a double strand break. This can then increase efficiency of targeted knock-out or knock-in events at this locus. Can be used with flanking regions of the speE gene (BBa_K4050017 & BBa_K4050018) to knock-out the speE gene in Synechococcus elongatus UTEX 2973.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |