Plasmid

Part:BBa_K4047036:Design

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-06)


pGEM-tal


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7968
    Illegal NheI site found at 2673
    Illegal NheI site found at 8658
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
    Illegal NotI site found at 515
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
    Illegal NgoMIV site found at 1043
    Illegal NgoMIV site found at 3454
    Illegal NgoMIV site found at 8493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 198
    Illegal BsaI site found at 2068
    Illegal BsaI site found at 9117
    Illegal BsaI.rc site found at 4839
    Illegal BsaI.rc site found at 5605
    Illegal SapI site found at 3756


Design Notes

Source

Gene sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for transaldolase, at the locus tag Synpcc7942_2297. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2367528&to=2368721&report=fasta&strand=2

Backbone sourced from pAM2991 created by Susan Golden in 2005 for work in S. elongatus PCC 7942.

https://www.addgene.org/40248/


References