Regulatory
KanR Promo

Part:BBa_K4047020

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-06)


Kanamycin Promoter for Synechococcus

This promoter allows the expression of kanamycin resistance (KanR) in Synechococcus elongatus PCC 7942, optimized for this photosynthetic chassis as opposed to other bacteria. Specifically, this part was created in conjunction with the kanamycin resistance coding sequence (BBa_K4047019) and the kanamycin terminator sequence (BBa_K4047021) as part of the plasmid BBa_K4047039.

This part was functional in wet lab experiments, allowing expression of functional kanamycin resistance and growth of transformed colonies on kanamycin-containing BG11 plates.

This sequence varies from an established promoter registered in 2006 (BBa_P1003) as part of a kanamycin cassette. This promoter is particularly well suited for strong expression in Synechococcus while retaining full BioBrick compatibility, offering more options to future iGEM teams. This part is linked as an improvement on the previous part page for those looking for a promoter better suited for photosynthetic species.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None