Coding
dSBPase- B

Part:BBa_K4047016:Design

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-04)


Gentamicin resistance (GmR) for Synechococcus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This resistance marker was contained in an established plasmid backbone used within the Miami University Wang microbiology lab.

The marker also shares 100% identity with Shuttle vector pXsp8, found at the following Genbank link (https://www.ncbi.nlm.nih.gov/nucleotide/KC817027.1?report=genbank&log$=nuclalign&blast_rank=1&RID=R0SPGK2Z013)

References