Primer
pTF_fbp-fw

Part:BBa_K4047005:Design

Designed by: An Nguyen, Avery Imes   Group: iGEM21_MiamiU_OH   (2021-09-07)


primerTF_fbp-foward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This primer was created using the information detailed below within the NEBuilder free online tool located at https://nebuilder.neb.com/#!/


Source

Generated from a combination of factors. Overlap sequence sourced from pAM2991. This sequence lies downstream of the trc promoter and is thus likely to contain a shine-dalgarno binding sequence, increasing the expression of fbp in the polycistronic mRNA produced. https://www.addgene.org/40248/

Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2

References