Part:BBa_K4047003

primerF/TF_fbp-reverse

Reverse primer for FBPase

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 14
  Illegal PstI site found at 2
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 2
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 20
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 14
  Illegal PstI site found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 14
  Illegal PstI site found at 2
  
• 1000
  COMPATIBLE WITH RFC[1000]

Long Description

This is the reverse primer to amplify the gene for fructose-1,6-bisphosphatase (BBa_K4047035). The overlap sequence allows proper Gibson assembly with the pGEM-fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.

This is also the reverse primer to amplify the gene for fructose-1,6-bisphosphatase prior to the assembly of pGEM-tal+fbp (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone, the same used for pGEM-fbp. The annealing portion (all caps) allows annealing to the wild type S. elongatus PCC 7942 genome for proper amplification.