Composite
Part:BBa_K4046750:Design
Designed by: Andy Qiao Group: iGEM21_Duke (2021-10-19)
CMV - BS #2 - Kozak - tdTomato - bghA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 998
Illegal PstI site found at 1724 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 998
Illegal PstI site found at 1724 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 614
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 998
Illegal PstI site found at 1724 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 998
Illegal PstI site found at 1724 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This gene was synthesized commercially from the published sequence (IDT). tdTomato is the modified product of a gene originally from mushroom corals in the Discosoma family. The gene was obtained through PCR of a commercially available plasmid pCRISPR-HOT_tdTomato (Addgene, 138567).
Source
This is an identified binding site for the DhdR gene, and is generated from sequence analysis.
References
A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR Dan Xiao, Wen Zhang, Xiaoting Guo, Yidong Liu, Chunxia Hu, Shiting Guo, Zhaoqi Kang, Xianzhi Xu, Cuiqing Ma, Chao Gao, Ping Xu bioRxiv 2021.02.18.430539; doi: https://doi.org/10.1101/2021.02.18.430539