Composite

Part:BBa_K4046740:Design

Designed by: Andy Qiao   Group: iGEM21_Duke   (2021-10-19)


CMV - BS #1 with spacer - BS #1 with spacer - BS#1 - Kozak - tdTomato - bghA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1052
    Illegal PstI site found at 1778
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1052
    Illegal PstI site found at 1778
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1052
    Illegal PstI site found at 1778
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1052
    Illegal PstI site found at 1778
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene was synthesized commercially from the published sequence (IDT). tdTomato is the modified product of a gene originally from mushroom corals in the Discosoma family. The gene was obtained through PCR of a commercially available plasmid pCRISPR-HOT_tdTomato (Addgene, 138567).


Source

This is an identified binding site for the DhdR gene, and is generated from sequence analysis.

References