Regulatory

Part:BBa_K4046200:Design

Designed by: Ashley Jones   Group: iGEM21_Duke   (2021-10-18)


DhdO Binding Site #2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is one of two versions of the dhdO binding site that was tested. Eventually, we hope to characterize the binding behavior of both sequences, in order to optimize our circuit behavior.


Source

This is an identified binding site for the DhdR gene, and is generated from sequence analysis. This gene was synthesized commercially from the published sequence (IDT).

References

A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR Dan Xiao, Wen Zhang, Xiaoting Guo, Yidong Liu, Chunxia Hu, Shiting Guo, Zhaoqi Kang, Xianzhi Xu, Cuiqing Ma, Chao Gao, Ping Xu bioRxiv 2021.02.18.430539; doi: https://doi.org/10.1101/2021.02.18.430539