Coding

Part:BBa_K4046010:Design

Designed by: Jessica Shah   Group: iGEM21_Duke   (2021-10-18)


DhdR-NLS-FLAG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 667
    Illegal PstI site found at 134
    Illegal PstI site found at 317
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 277
    Illegal PstI site found at 134
    Illegal PstI site found at 317
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 351
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 667
    Illegal PstI site found at 134
    Illegal PstI site found at 317
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 667
    Illegal PstI site found at 134
    Illegal PstI site found at 317
    Illegal NgoMIV site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 213


Design Notes

Note that this version of the protein is not compatible with BioBrick standards; for a modified version of the gene that is BioBrick compatible, please consult part BBa_K4046000.

Source

DhdR is a transcriptional repression factor isolated from the bacteria Achromobacter denitrificans .

References

Xiao, D., Zhang, W., Guo, X., Liu, Y., Hu, C., Guo, S., Kang, Z., Xu, X., Ma, C., Gao, C., & Xu, P. (2021). A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DHDR. https://doi.org/10.1101/2021.02.18.430539