Coding

Part:BBa_K4046000:Design

Designed by: Jessica Shah   Group: iGEM21_Duke   (2021-10-18)


FLAG-NLS-DhdR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 310
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 186
    Illegal BsaI site found at 303


Design Notes

This gene was synthesized commercially from the published sequence (Twist Biosciences). Further testing will be run to determine which order of sequence leads to optimal DhdR expression. This would include running a Western blot in order to visualize expression levels of the protein within the cell.

Source

DhdR is a transcriptional repression factor isolated from the bacteria Achromobacter denitrificans .

References

Xiao, D., Zhang, W., Guo, X., Liu, Y., Hu, C., Guo, S., Kang, Z., Xu, X., Ma, C., Gao, C., & Xu, P. (2021). A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DHDR. https://doi.org/10.1101/2021.02.18.430539