Coding

Part:BBa_K4044003:Design

Designed by: Kalinichenko Andrei   Group: iGEM21_LMSU   (2021-10-19)


BcLOV4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 354
    Illegal PstI site found at 598
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 354
    Illegal PstI site found at 598
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 354
    Illegal PstI site found at 598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 354
    Illegal PstI site found at 598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization of the nucleotide sequence for efficient gene expression in E. coli was performed using GENEWIZ codon optimization tool.


Source

BcLOV4 protein sequence was taken from https://www.uniprot.org/uniprot/A0A384J9J8 and codon optimized for E. coli.

Source organism: Botrytis cinerea

References