Composite

Part:BBa_K4040021:Design

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-27)


Synthetic Receptor IL6R-TCS-Gal4-KRAB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1237
    Illegal PstI site found at 1414
    Illegal PstI site found at 1969
    Illegal PstI site found at 2815
    Illegal PstI site found at 2974
    Illegal PstI site found at 3082
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1237
    Illegal PstI site found at 1414
    Illegal PstI site found at 1969
    Illegal PstI site found at 2815
    Illegal PstI site found at 2974
    Illegal PstI site found at 3082
    Illegal NotI site found at 3323
    Illegal NotI site found at 3944
    Illegal NotI site found at 4256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2692
    Illegal BamHI site found at 3825
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1237
    Illegal PstI site found at 1414
    Illegal PstI site found at 1969
    Illegal PstI site found at 2815
    Illegal PstI site found at 2974
    Illegal PstI site found at 3082
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1237
    Illegal PstI site found at 1414
    Illegal PstI site found at 1969
    Illegal PstI site found at 2815
    Illegal PstI site found at 2974
    Illegal PstI site found at 3082
    Illegal NgoMIV site found at 796
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 892
    Illegal NgoMIV site found at 904
    Illegal NgoMIV site found at 1754
    Illegal NgoMIV site found at 1889
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N


Source

N

References