Coding

Part:BBa_K4040008

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-02)


αS-scFv

The αS-scFv, also named CR3022, is a scFv derived from an antibody recognizing the spike protein of the SARS-CoV-2. As the previous research reported, the αS-scFv has a striking affinity towards the S protein. We consequently adopted the αS-scFv to be the extracellular domain of our CARs to improve the phagocytosis efficiency of our CAR-macrophages.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
    Illegal PstI site found at 640
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
    Illegal PstI site found at 640
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
    Illegal PstI site found at 640
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
    Illegal PstI site found at 640
    Illegal NgoMIV site found at 352
    Illegal NgoMIV site found at 688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 568


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Categories
Parameters
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