Plasmid

Part:BBa_K4034010:Design

Designed by: Spyros Kanellopoulos   Group: iGEM21_Athens   (2021-10-17)


pPfusionT7, plasmid that overexpresses Pfusion polymerase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3145
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 3139
    Illegal EcoRI site found at 3198
    Illegal EcoRI site found at 3798
    Illegal PstI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 3139
    Illegal EcoRI site found at 3198
    Illegal EcoRI site found at 3798
    Illegal NheI site found at 2420
    Illegal SpeI site found at 3146
    Illegal PstI site found at 234
    Illegal PstI site found at 3160
    Illegal NotI site found at 3153
    Illegal NotI site found at 3209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 3139
    Illegal EcoRI site found at 3198
    Illegal EcoRI site found at 3798
    Illegal BglII site found at 998
    Illegal BamHI site found at 290
    Illegal BamHI site found at 1073
    Illegal XhoI site found at 284
    Illegal XhoI site found at 3203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3146
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 3139
    Illegal EcoRI site found at 3198
    Illegal EcoRI site found at 3798
    Illegal PstI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 3139
    Illegal EcoRI site found at 3198
    Illegal EcoRI site found at 3798
    Illegal SpeI site found at 3146
    Illegal PstI site found at 234
    Illegal PstI site found at 3160
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

No

References