Composite

Part:BBa_K4023003:Design

Designed by: Jiachen Lin   Group: iGEM21_Washington   (2021-10-21)


glpF-rbs-modified MTIA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 183


Design Notes

Literature review showed strong evidence that transport of arsenite into bacteria often limits remediation capacity of the whole remediator. Hence when considering choice of protein channels, we set parameters to find a transport protein that is widely used in E.coli and is specific to arsenite. When designing the composite part, we consulted with our mentor on how to best express a bicistronic gene and she recommended adding a RBS between the glpF sequence and modified MTIA sequence.


Source

glpF codes for the transmembrane glycerol uptake facilitator protein found in E.coli strain K12: https://parts.igem.org/Part:BBa_K190028 RBS: https://pubmed.ncbi.nlm.nih.gov/27463675/ Metallothionein-IA is a protein derived from Homo sapiens. The gene coding for metallothionein-IA is MTIA and is found within chromosome 16: https://www.uniprot.org/uniprot/P04731

References