Part:BBa_K4020006

Usage and Biology

The DNA sequence encodes for VP16, a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early (IE) genes (HIRAI et al., 2010). The N-terminal region of the protein confers specificity for the IE genes, whilst the C-terminal part contains two functional regions, VP16ad/c and VP16ad/n, that are required for transcriptional activation (Jonker et al., 2005). The residues composing the VP16ad/c subdomain are from 454 to 490. Both subdomains interact with the transcriptional coactivator PC4 and the general transcription factor TFIIBc. Glutamate 476 plays a crucial role in transcriptional activation (Jonker et al., 2005). It was acquired from the DualMembrane Kit 3 (Thaminy et al., 2003).

References

• HIRAI, H., TANI, T., & KIKYO, N. (2010). Structure and functions of powerful transactivators: VP16, MyoD and FoxA. The International Journal of Developmental Biology, 54(11–12), 1589. https://doi.org/10.1387/IJDB.103194HH
• Jonker, H. R. A., Wechselberger, R. W., Boelens, R., Folkers, G. E., & Kaptein, R. (2005). Structural Properties of the Promiscuous VP16 Activation Domain. Biochemistry, 44(3), 827–839. https://doi.org/10.1021/bi0482912
• Thaminy, S., Auerbach, D., Arnoldo, A., & Stagljar, I. (2003). Identification of Novel ErbB3-Interacting Factors Using the  Split-Ubiquitin Membrane Yeast Two-Hybrid System. Genome Research, 13(7), 1744. https://doi.org/10.1101/GR.1276503