Part:BBa_K4020005

Usage and Biology

The DNA sequence encodes for VP16, a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early (IE) genes (HIRAI et al., 2010). The N-terminal region of the protein confers specificity for the IE genes, whilst the C-terminal part contains two functional regions, VP16ad/c and VP16ad/n, that are required for transcriptional activation (Jonker et al., 2005). The residues composing the VP16ad/n subdomain are from 412 to 453. Both subdomains interact with the transcriptional coactivator PC4 and the general transcription factor TFIIBc to promote transcriptional activation (Jonker et al., 2005). It was acquired from the DualMembrane Kit 3 (Thaminy et al., 2003).

References

• HIRAI, H., TANI, T., & KIKYO, N. (2010). Structure and functions of powerful transactivators: VP16, MyoD and FoxA. The International Journal of Developmental Biology, 54(11–12), 1589. https://doi.org/10.1387/IJDB.103194HH
• Jonker, H. R. A., Wechselberger, R. W., Boelens, R., Folkers, G. E., & Kaptein, R. (2005). Structural Properties of the Promiscuous VP16 Activation Domain. Biochemistry, 44(3), 827–839. https://doi.org/10.1021/bi0482912
• Thaminy, S., Auerbach, D., Arnoldo, A., & Stagljar, I. (2003). Identification of Novel ErbB3-Interacting Factors Using the  Split-Ubiquitin Membrane Yeast Two-Hybrid System. Genome Research, 13(7), 1744. https://doi.org/10.1101/GR.1276503