Coding
Part:BBa_K4020001
Designed by: Karina Kurbanova Group: iGEM21_Estonia_TUIT (2021-10-18)
Usage and Biology
LexA Binding Domain is a sequence in the LexA protein, a transcriptional repressor (Zhang et al., 2010) of the SOS regulon in Escherichia coli that allows the binding of different regulatory molecules (Thliveris & Mount, 1992). It was acquired from the DualMembrane Kit 3 (Thaminy et al., 2003).
References
- Thliveris, A. T., & Mount, D. W. (1992). Genetic identification of the DNA binding domain of Escherichia coli LexA protein. Proceedings of the National Academy of Sciences, 89(10), 4500–4504. https://doi.org/10.1073/PNAS.89.10.4500
- Zhang, A. P. P., Pigli, Y. Z., & Rice, P. A. (2010). Structure of the LexA-DNA complex and implications for SOS box measurement. Nature, 466(7308), 883. https://doi.org/10.1038/NATURE09200
- Thaminy, S., Auerbach, D., Arnoldo, A., & Stagljar, I. (2003). Identification of Novel ErbB3-Interacting Factors Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System. Genome Research, 13(7), 1744. https://doi.org/10.1101/GR.1276503
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Categories
Parameters
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