Composite

Part:BBa_K4015015:Design

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-21)


Lcp1VH2-HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 508
    Illegal XhoI site found at 2033
    Illegal XhoI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 485
    Illegal NgoMIV site found at 1679
    Illegal NgoMIV site found at 2001
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 112
    Illegal BsaI.rc site found at 1582
    Illegal SapI.rc site found at 100


Design Notes

The indication has to be included in the product, if not the secretion cannot be detect.


Source

The parts are assembled through Gibson assembly, and the product is ligated in pET23a.

References