Composite

Part:BBa_K4015005:Design

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-19)


NusA-Lcp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1377
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The binding of NusA to Lcp can increase the solubility of Lcp VH2 which can increase its yielding while binding to the Lcp protein functional group affect its function and reduce its oxidation and degradation capacity.


Source

The NusA-Lcp was ligated into PET23a.

References