Composite

Part:BBa_K4011014:Design

Designed by: Meilun Wu   Group: iGEM21_LINKS_China   (2021-10-21)


pTac-RiboJ-TnaA-FL-FMO-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4158
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2565
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. All codons were optimized for E. coli<.i> based on <i>E. coli codon bias.

2. The sequences for the flexible linker is GGGGSGGGGS.

3. Transformed and expressed in E. coli DH5α ΔTnaA to negate influence of endogenous TnaA in measurements.


Source

TnaA-FL-FMO is composed of two main domains: tryptophanase (TnaA) from E. coli and flavin-containing monooxygenase (FMO) from Methylophaga aminisulfidivorans.

References