Coding

Part:BBa_K4011009:Design

Designed by: Zixiang Zhou   Group: iGEM21_LINKS_China   (2021-10-21)


CBM2-NT2RepCT-CBM2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 52
    Illegal AgeI site found at 96
    Illegal AgeI site found at 268
    Illegal AgeI site found at 334
    Illegal AgeI site found at 1444
    Illegal AgeI site found at 1488
    Illegal AgeI site found at 1660
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. All codons were optimized for E. coli based on E. coli codon bias

2. Flexible linker GSGGGS used between first CBM2 and NT2RepCT, flexible linker GGGGS used between NT2RepCT and second CBM2.

3. 6x histag (MGHHHHHHM) added to C-terminal to allow for Ni-NTA purification.



Source

CBM2 is derived from Cellulomonas fimi.. NT2RepCT is an artificial spider silk fibroin (Visit BBa_K3264000 for more).


References