Composite
Part:BBa_K4006013:Design
Designed by: Margaret Cook Group: iGEM21_ASU (2021-10-21)
MT-6XHis-IEE5-arsC-HA-IEE2-FLAG-fTNa
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 589
Illegal BamHI site found at 45 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 5
Illegal SapI site found at 685
Illegal SapI site found at 1750
Illegal SapI.rc site found at 233
Illegal SapI.rc site found at 1150
Illegal SapI.rc site found at 2305
Design Notes
This part was designed for Golden Gate assembly and use with the type IIS restriction enzyme SapI in the compatible plasmid backbone pASapI. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii.
Source
Part BBa_K3275000 - Human MT sequence
10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin IEE2
Uniprot ID P08692 - E.coli ArsC sequence
10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin IEE5
Uniprot FTNA_ECOLI P0A998 - E.coli FtnA sequence
BBa_K2207020 - FLAG tag and linker