Composite

Part:BBa_K4006013:Design

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-21)


MT-6XHis-IEE5-arsC-HA-IEE2-FLAG-fTNa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 589
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5
    Illegal SapI site found at 685
    Illegal SapI site found at 1750
    Illegal SapI.rc site found at 233
    Illegal SapI.rc site found at 1150
    Illegal SapI.rc site found at 2305


Design Notes

This part was designed for Golden Gate assembly and use with the type IIS restriction enzyme SapI in the compatible plasmid backbone pASapI. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii.

Source

Part BBa_K3275000 - Human MT sequence
10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin IEE2
Uniprot ID P08692 - E.coli ArsC sequence
10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin IEE5
Uniprot FTNA_ECOLI P0A998 - E.coli FtnA sequence
BBa_K2207020 - FLAG tag and linker

References