Composite
Part:BBa_K4006009:Design
Designed by: Margaret Cook Group: iGEM21_ASU (2021-10-21)
MT-IEE5-arsC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 606
Illegal BamHI site found at 40 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 224
Illegal SapI.rc site found at 31
Design Notes
This part was designed for Golden Gate assembly and use with the type IIS restriction enzyme SapI in the compatible plasmid backbone pASapI. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii
Source
Part BBa_K3275000 - Human MT sequence 10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin IEE5 Uniprot ID P08692 - E.coli ArsC sequence