Composite

Part:BBa_K4006009:Design

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-21)


MT-IEE5-arsC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 606
    Illegal BamHI site found at 40
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 224
    Illegal SapI.rc site found at 31


Design Notes

This part was designed for Golden Gate assembly and use with the type IIS restriction enzyme SapI in the compatible plasmid backbone pASapI. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii

Source

Part BBa_K3275000 - Human MT sequence 10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin IEE5 Uniprot ID P08692 - E.coli ArsC sequence

References