Regulatory
Part:BBa_K4006007:Design
Designed by: Margaret Cook Group: iGEM21_ASU (2021-10-21)
IEE2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
IEE2 should only be utilized when the upstream and downstream flanking elements of the plasmid backbone are a significant distance from the the psbN-psbH junction.
Source
10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin
References
Macedo-Osorio, K.S., Pérez-España, V.H., Garibay-Orijel, C. et al. Intercistronic expression elements (IEE) from the chloroplast of Chlamydomonas reinhardtii can be used for the expression of foreign genes in synthetic operons. Plant Mol Biol 98, 303–317 (2018). https://doi.org/10.1007/s11103-018-0776-z