Coding

Part:BBa_K4006005:Design

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-20)


arsC-HA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5
    Illegal SapI.rc site found at 470


Design Notes

This part was designed for Golden Gate assembly and use with the type IIS restriction enzyme SapI in the compatible plasmid backbone pASapI. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii. The HA tag was added to the N terminal of the protein coding sequence to ensure there was no interaction with the functional domain of the folded protein.

Source

Uniprot ID P08692 - E.coli ArsC sequence

References