Translational_Unit

Part:BBa_K3994011:Design

Designed by: Nixue Song   Group: iGEM21_NOFLS_YZ   (2021-10-12)


PyeaR_HrpR+PttB344_HrpS_PJ23105_ttrR+PhrpL_amilGFP_IL10_PJ23104_ttrS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 1398
    Illegal XbaI site found at 3288
    Illegal XbaI site found at 3438
    Illegal PstI site found at 1236
    Illegal PstI site found at 3276
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2062
    Illegal NheI site found at 2085
    Illegal NheI site found at 5496
    Illegal NheI site found at 5519
    Illegal PstI site found at 1236
    Illegal PstI site found at 3276
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3116
    Illegal BamHI site found at 1254
    Illegal BamHI site found at 3294
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 1398
    Illegal XbaI site found at 3288
    Illegal XbaI site found at 3438
    Illegal PstI site found at 1236
    Illegal PstI site found at 3276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 1398
    Illegal XbaI site found at 3288
    Illegal XbaI site found at 3438
    Illegal PstI site found at 1236
    Illegal PstI site found at 3276
    Illegal NgoMIV site found at 669
    Illegal AgeI site found at 142
    Illegal AgeI site found at 256
    Illegal AgeI site found at 262
    Illegal AgeI site found at 901
    Illegal AgeI site found at 1776
    Illegal AgeI site found at 2329
    Illegal AgeI site found at 2335
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

No

References