Coding

Part:BBa_K3991001

Designed by: Ke ZHU   Group: iGEM21_Shanghai_United   (2021-10-12)


ArsR

Profile

Name: ArsR

Base Pairs: 351 bp

Origin: Escherichia coli str. K-12 substr. MG1655

Properties: Gene technology for protecting patented bacterial strains

ArsR negatively controls the expression of the genes involved in arsenical and antimonite metals resistance whose expression is induced in the presence of heavy metals ，which is a trans-acting regulatory protein which controls its own expression. The repressive effect of ArsR is alleviated by oxyions of +III oxidation state of arsenic, antimony, and bismuth, as well as arsenate (As(V)).

Experimental approach

Production, purification, and sequcing analysis of recombinant ArsR-amilGFP

Figure 1: Gel Electrophoresis Results of PCR of amilGFP Genes..

Figure 1 shows gel electrophoresis results of amilGFP PCR. Column M is a 2K marker ladder. Columns 1-6 are PCR products of amilGFP genes. All 1-6 columns displayed successful results at 700bp which could be used for later experiments. 

Figure 2: Gel Electrophoresis Results of PCR of ArsR Genes..

Figures2 show the result for colony PCR identification on the E.coli with ArsR inserted that were cultivated previously. The purpose is to examine whether the E.coli contains expected gene segment of ARSR.

Figure 3..

References

(1)Neff, J.M. (1997). ECOTOXICOLOGY OF ARSENIC IN THE MARINE ENVIRONMENT—Review. Environmental Toxicology and Chemistry, 16(5), p.917.

(2)Chinese Center For Disease Control and Prevention (2014). 中国疾病预防控制中心. [online] www.chinacdc.cn.

(3)Ahmad, S. A., Khan, M. H., & Haque, M. (2018, November 30). Arsenic contamination in groundwater in Bangladesh: Implications and challenges for healthcare policy. Risk management and healthcare policy. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281155/.

(4)Argos, M. (2012, December 1). Arsenic and human health: epidemiologic progress and public health implications. De Gruyter. https://www.degruyter.com/document/doi/10.1515/reveh-2012-0021/html

(5)Arsenic. (2021, May 3). National Institute of Environmental Health Sciences. https://www.niehs.nih.gov/health/topics/agents/arsenic/index.cfm

(6)Institute, E. (2020, May 6). Clay layers and Distant PUMPING Trigger arsenic contamination in Bangladesh Groundwater. State of the Planet. https://news.climate.columbia.edu/2020/05/07/clay-arsenic-bangladesh-groundwater/.

(7)International Agency for Research on Cancer. (2012). Review of Human Carcinogens: C. Metals, Arsenic, Dusts and Fibres (IARC Monographs on the Evaluation of the Carcinogenic Risks to Humans, 100) (Vol. 100C). World Health Organization. https://publications.iarc.fr/120

(8)Matta, G. (2016, June). 2015 - 2016_Mercury, lead and arsenic impact on environment and human health.pdf. Academia.Edu. https://www.academia.edu/38166988/2015_2016_Mercury_lead_and_arsenic_impact_on_environment_and_human_health_pdf

(9)Saha, J. C., Dikshit, A. K., Bandyopadhyay, M. A., & Saha, K. C. (1999, July 1). A Review of Arsenic Poisoning and its Effects on Human Health. ResearchGate. https://www.researchgate.net/publication/248944528_A_Review_of_Arsenic_Poisoning_and_its_Effects_on_Human_Health

(10)SUI Jiachen, YU Hansong, DAI jiayu, et al. Advances in the application of biosensor technology for the detection of heavy metal arsenic in foods[J]. Food Science, 2016, 37(7): 233-238. DOI:10.7506/spkx1002-6630-201607042. http://www.spkx.net.cn

(11)Shaji, E., Santosh, M., Sarath, K., Prakash, P., Deepchand, V., & Divya, B. (2021). Arsenic contamination of groundwater: A global synopsis with focus on the Indian Peninsula. Geoscience Frontiers, 12(3). https://doi.org/10.1016/j.gsf.2020.08.015

(12)The American Cancer Society medical and editorial content team. (2020, August 5). Arsenic and Cancer Risk. American Cancer Society. https://www.cancer.org/cancer/cancer-causes/arsenic.html

(13)Undark Magazine. (2019, December 20). The Poisoning of Bangladesh: How Arsenic Is Ravaging a Nation. https://undark.org/2017/08/16/bangladesh-arsenic-poisoning-drinking-water/

(14)Yogarajah, N., & Tsai, S. S. H. (2015, May 1). Detection of trace arsenic in drinking water: challenges and opportunities for microfluidics - Environmental Science: Water Research & Technology (RSC Publishing) DOI:10.1039/C5EW00099H. Royal Society of Chemistry. https://pubs.rsc.org/en/content/articlehtml/2015/ew/c5ew00099h

(15)Arsenic W.H.O. World Health Organization. February. 2018. [Accessed August 3, 2018]. Available from: http://www.who.int/news-room/fact-sheets/detail/arsenic.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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