Coding
Part:BBa_K3990015:Design
Designed by: Li Wenxi Group: iGEM21_SMS_Shenzhen (2021-10-01)
RoxB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1937
Illegal BamHI site found at 186
Illegal BamHI site found at 1386 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1078
Illegal NgoMIV site found at 1429
Illegal NgoMIV site found at 1465
Illegal AgeI site found at 598 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Rubber cleavage enzyme, the rubber oxygenase RoxBXsp (RoxB of Xanthomonas sp. strain 35Y)
Source
From Xanthomonas sp. strain 35Y.