Measurement
Part:BBa_K398503:Design
Designed by: Mathias Voges Group: iGEM10_TU_Delft (2010-07-12)
J23100 J61117 E0040 B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
Design Notes
Beware of an additional XbaI site between J23100 and J61117. Its presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is forward insert friendly (EcoRI, SpeI).
Source
iGEM 2010 distribution plates.