Measurement
Part:BBa_K398331:Experience
Designed by: Kira Schipper Group: iGEM10_TU_Delft (2010-10-07)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K398331
- iGEM TU Delft 2010
In order to measure the strength (efficiency) of the pCaiF promoter we built the part K938331 which is pCaiF+ E0240. Check [http://2010.igem.org/Team:TU_Delft#page=Project/sensing/results our results page] in order to know more about our experience with this part!!!
| Condition | Exponential phase [GFP molecules/O.D.] | Stationary phase [GFP molecules/O.D.] |
|---|---|---|
| LB | 1.2516E+07 | -8.6245E+05 |
| 0.5LB | 1.8101E+07 | -2.5945E+05 |
| [glc]=10g/L | 6.6456E+06 | -6.7109E+06 |
| [glc]=5g/L | 8.3673E+06 | 4.2339E+06 |
| [glc]=2g/L | 7.2869E+06 | 3.9755E+07 |
| Diauxic growth (glucose phase) | 7.4517E+06 | 2.1543E+07 |
| Diauxic growth (Laurate phase) | 1.1435E+07 | 6.6428E+06 |
Also a simple model for this system was developed. [http://2010.igem.org/Team:TU_Delft#page=Modeling/pcaif-model Click here] in order to know more about it!.
User Reviews
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