Plasmid

Part:BBa_K3978048:Design

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


reget-plasmid-cIcIDN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal XbaI site found at 5457
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 112
    Illegal PstI site found at 131
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 112
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal XbaI site found at 5457
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal XbaI site found at 5457
    Illegal PstI site found at 131
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 671
    Illegal NgoMIV site found at 5348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1256
    Illegal SapI.rc site found at 2916


Design Notes

When adding the ssrA tag to protein CIDN, it is careful not to influence the folding process of CIDN. Adding a short sequence between the tad and the sequence is advisable. The sequence of CIDN should precede the CI.


Source

This part is designed by ourselves.

References