Coding

Part:BBa_K3978003:Design

Designed by: Ding Haote   Group: iGEM21_HiZJU-China   (2021-10-21)


ERa-421F


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 403
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 286
    Illegal BamHI site found at 769
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 403
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 403
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Insert the targeted gene ERa-421F between NdeI and BamHI


Source

The sequence comes from PDB. And we changed the site 421 M to F.

References