Measurement
Part:BBa_K3971024:Design
Designed by: Misaal Bedi Group: iGEM21_IISER-Pune-India (2021-10-19)
Promoter characterization cassette for native E.coli csc operon bidirectional promoter.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1699
Design Notes
The double terminator downstream of the BFP gene had to reverse complemented (reverse complement of BBa_B0015) and the BFP gene had to be reverse complemented as well (reverse complement of BBa_K592100) since this is a bidirectional promoter, and these sequences had to be transcribed on the strand opposite to the strand with YFP.
Source
The YFP and the double terminators (coding in both directions) are present in the iGEM 2021 Distribution Kit. We plan to get the BFP gene synthesized. The promoter can be amplified from the pCSCX plasmid, which was a gift from Claudia Vickers (Addgene plasmid # 63918 ; http://n2t.net/addgene:63918 ; RRID:Addgene_63918).