Measurement

Part:BBa_K3971020

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-10-11)


Promoter characterization cassette for native E.coli csc operon bidirectional promoter.

This composite part has been designed to characterize the activity of the native E.coli csc operon bidirectional promoter.

Figure of the composite construct for characterizing cscB transport efficiencies under different promoters. NS1a and NS1b are the two homology arms to Neutral Site 1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1478


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Parameters
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