Composite

Part:BBa_K396000:Design

Designed by: Ryuji Yamamoto   Group: iGEM10_Chiba   (2010-10-21)


T7/CI Hybrid Promoter with GFP (composite with LuxR generator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 783
    Illegal NheI site found at 806
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 852
    Illegal BamHI site found at 1421
    Illegal XhoI site found at 822
    Illegal XhoI site found at 1322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LuxR generator functions as it is designed. T7/CI hybrid promoter function is checked by using BL21(DE3) strain, resulted in that it works within limited range of the expression of T7 RNA polymerase (IPTG concentration<100μM).


Source

Lambda CI operator sequence (OR1, BBa_R1051) is connected to T7 Promoter (BBa_I719005). LuxR generator is consisted of constitutive promoter (BBa_J23100) and LuxR coding sequence (BBa_C0062).


References