Reporter

Part:BBa_K3957001:Design

Designed by: Shamitha Aravind   Group: iGEM21_Northern_BC   (2021-09-11)


Composite part consisting of promoter, RBS, terminator, and part designed to detect 1,25-dihydroxyvi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 932
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K3957000 was codon-optimized for E. coli. Restriction sites for EcoRI and XbaI were added at the beginning of the sequence, and a site for SpeI was added at the end.


Source

Composite part; BBa_K3957000 is a modified version of the part BBa_K3515011.


References