DNA
Part:BBa_K3956004:Design
Designed by: Chris Jung Group: iGEM21_CCA_San_Diego (2021-07-31)
5'-UTR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Although 5'UTR can be completely missing in leaderless mRNA, it is usually included without too much consideration.
Source
Decoene, T., De Maeseneire, S. L., & De Mey, M. (2019). Modulating transcription through development of semi-synthetic yeast core promoters. PloS one, 14(11), e0224476. https://doi.org/10.1371/journal.pone.0224476