Part:BBa_K3955103
DB3DB3 peptide fused with L. lactis Ups45 signal peptide
DB3DB3 is a peptide designed for inhibition of amyloid beta aggregation [1]. It has also shown to have inhibitory effects on curli fibre elongation [2].
Usp45 originates in Lactococcus lactis and is a major extracellular component [3]. Its secretion signal peptide has been shown to successfully cause secretion in L. reuteri when fused with GFP [4].
References
1. Klein A, Ziehm T, Tusche M, Buitenhuis J, Bartnik D, Boeddrich A et al. Optimization of the All-D Peptide D3 for Aβ Oligomer Elimination. PLOS ONE. 2016;11(4):e0153035.
2. Perov S, Lidor O, Salinas N, Golan N, Tayeb- Fligelman E, Deshmukh M et al. Structural Insights into Curli CsgA Cross-β Fibril Architecture Inspire Repurposing of Anti-amyloid Compounds as Anti-biofilm Agents. PLOS Pathogens. 2019;15(8):e1007978.
3. van Asseldonk M, de Vos W, Simons G. Functional analysis of the Lactococcus lactis usp45 secretion signal in the secretion of a homologous proteinase and a heterologous α-amylase. Molecular and General Genetics MGG. 1993;240(3):428-434.
4. Wu C, Chung T. Green fluorescent protein is a reliable reporter for screening signal peptides functional in Lactobacillus reuteri. Journal of Microbiological Methods. 2006;67(1):181-186.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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