Coding
csgF pepti

Part:BBa_K3955050

Designed by: Jacob Miefalk   Group: iGEM21_Lund   (2021-09-30)


Repeat of CsgF peptide blocking CsgG mutant

The part is a repeat of CsgF mutant peptide blocking the CsgG pore, originally called PeptideNN17R. csgF and csgG are part of the curli secretion system. [1] This part can be cleaved to make several CsgF peptide mutants that can block the pore of the CsgG/CsgF complex that secreate CsgA which form curli.

References 1. Zhaofeng Yan, Meng Yin, Jianan Chen, Xueming Li. Assembly and substrate recognition of curli biogenesis system. Nature Communications [Internet]. 2020 Jan 1 [cited 2021 Oct 9];11(1):1–10. Available from: https://search.ebscohost.com/login.aspx?direct=true&db=edsdoj&AN=edsdoj.19f841225ae740398e4682f0391a5534&site=eds-live&scope=site

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 160
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None