Other

Part:BBa_K3947005:Design

Designed by: David Dimech   Group: iGEM21_MSP-Maastricht   (2021-10-19)


Spacer System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 31
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ensuring that there is a restriction site at both spacer locations.


Source

This sequence was retrieved from pET39b(+).

References