Coding

Part:BBa_K3946023:Design

Designed by: Ritupreet Kaur   Group: iGEM21_Edinburgh   (2021-10-19)


PETase (W159H/ S238F)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No design considerations.


Source

The source organism is Ideonella sakaiensis 201-F6 and double mutant was obtained from Dr Joanna Sadler created with reference to https://doi.org/10.1073/pnas.1718804115

References

https://doi.org/10.1073/pnas.1718804115