Coding

Part:BBa_K3945004:Design

Designed by: Ramin Kahidi   Group: iGEM21_Calgary   (2021-10-20)


Probabilistic Mean Optimized Mutated Lanmodulin (PMO LanM)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 111
    Illegal BamHI site found at 186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation was gained by using the dataset created for the Efhe Neural network. From the large dataset of EF hands we were able to identify a probabilistic ‘average’ EF hand. This could be considered the mean of the EF hand protein subspace. The protein selected was chosen due to it having the most common amino acid for each indice. This mutant poses an improvement on the WT protein as it has a higher likelihood of binding an additional lanthanide in its otherwise unable fourth pocket.