Coding

Part:BBa_K3945002:Design

Designed by: Ramin Kahidi   Group: iGEM21_Calgary   (2021-10-20)


FC-Mutated Lanmodulin (FC LanM)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 111
    Illegal BamHI site found at 186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Family conservation mutated lanmodulin was generated using a brute force exploration into mutants that were similar in structure and charge distribution but different in the particular amino acids used in each. This was conducted by comprehensively generating sequences for the fourth pocket composed of amino acids that are within the same family of the wildtype lanmodulin. This identified if the interactions causing the unviable binding pocket were due to amino acids structure, not charge distribution.