![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3944034:Design
Designed by: Olof Dahlman Group: iGEM21_Chalmers-Gothenburg (2021-09-30)
zif268-ER LDB-VP16
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 166
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence must be assembled in a scarless fashion as the intended function relies on complete protein fusion. The protein must be present in a cell in order for estradiol induction using Z3BS repeats to be possible.
Source
Assembly provided by systems biology at Chalmers technical highschool.
References
1: R. Scott McIsaac, Patrick A. Gibney, Sunil S. Chandran, Kirsten R. Benjamin, David Botstein, Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae, Nucleic Acids Research, Volume 42, Issue 6, 1 April 2014, Page e48, https://doi.org/10.1093/nar/gkt1402