Coding
Part:BBa_K3944034
Designed by: Olof Dahlman Group: iGEM21_Chalmers-Gothenburg (2021-09-30)
zif268-ER LDB-VP16
Fusion protein consisting of protein zif268 which binds to repeats of the Z3BS recognition sequence, estradiol-recognizing domain ER LDB which triggers conformational change and activity upon binding to estradiol, and viral transcription protein VP16, transcribing genes downstream fusion protein upon binding to DNA sequence. The whole complex will trigger transcription of genes downstream of the Z3BS repeats upon addition of the induction agent estradiol in a dose-dependent manner (1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 166
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |