Coding

Part:BBa_K3944033:Design

Designed by: Olof Dahlman   Group: iGEM21_Chalmers-Gothenburg   (2021-09-30)


ScGAL1 + Z3BS-FatB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 915
    Illegal BsaI.rc site found at 1285
    Illegal BsaI.rc site found at 1758
    Illegal SapI site found at 734


Design Notes

May be assembled with scars in a biobrick compatible manner. Seven Z3BS repeats ensures optimal binding affinity. Z3BS repeats + ScGAL1 obtained as a single unit and cloned/assembled as such. FatB sequence was optimized for use in S.Cerevisiae.


Source

Assembled from parts obtained from IDT and systems biology at Chalmers technical highschool.

References

1: Joaquı́n J Salas, John B Ohlrogge, Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases, Archives of Biochemistry and Biophysics, Volume 403, Issue 1, 2002, Pages 25-34, ISSN 0003-9861, https://doi.org/10.1016/S0003-9861(02)00017-6.
2: Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae R. Scott McIsaac, Sanford J. Silverman, Megan N. McClean, Patrick A. Gibney, Joanna Macinskas, Mark J. Hickman, Allegra A. Petti, and David Botstein Molecular Biology of the Cell 2011 22:22, 4447-4459
3: R. Scott McIsaac, Patrick A. Gibney, Sunil S. Chandran, Kirsten R. Benjamin, David Botstein, Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae, Nucleic Acids Research, Volume 42, Issue 6, 1 April 2014, Page e48, https://doi.org/10.1093/nar/gkt1402