Part:BBa_K3944032:Design

ScGAL1+Z3BS - GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 169
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1223
  

Design Notes

May be assembled with biobrick scars since it is not a fusion protein. Seven Z3BS repeats ensure high binding affinity.

Source

Assembled from parts from IDT and systems biology at Chalmers technical highschool.

References

1: Rodriguez EA, Campbell RE, Lin JY, et al. The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins. Trends Biochem Sci. 2017;42(2):111-129. doi:10.1016/j.tibs.2016.09.010
2: Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae R. Scott McIsaac, Sanford J. Silverman, Megan N. McClean, Patrick A. Gibney, Joanna Macinskas, Mark J. Hickman, Allegra A. Petti, and David Botstein Molecular Biology of the Cell 2011 22:22, 4447-4459
3: R. Scott McIsaac, Patrick A. Gibney, Sunil S. Chandran, Kirsten R. Benjamin, David Botstein, Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae, Nucleic Acids Research, Volume 42, Issue 6, 1 April 2014, Page e48, https://doi.org/10.1093/nar/gkt1402